active+pro Caspase 3 Rabbit Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY1675
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active+pro
Caspase 3 Rabbit Monoclonal Antibody
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50μl
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618.00元
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來(lái)源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IP, IHC
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H
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35.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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-
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1:50-1:200
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-
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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active+pro
Caspase 3 Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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836(Human)
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SwissProt
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P42574(Human)
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Synonyms
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CPP32; CPP32B;
SCA-1
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Category
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Apoptosis
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Background
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Caspase-3,
also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific
cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is
expressed in cells as an inactive precursor from which the p17 and p11
subunits of the mature caspase-3 are proteolytically generated during
apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to
produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is
cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active
caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits.
At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an
Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated
caspase-3 releases SREBP from the membrane of the ER in a proteolytic
reaction that is distinct from their normal sterol-dependent activation.
Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix
leucine zipper domain and the membrane attachment domain. Caspase-3 also
cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes
a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver,
kidney and cells of the immune system.
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY1675
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active+pro
Caspase 3 Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說(shuō)明書
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1份
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保存條件:
active+pro
Caspase 3 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過(guò)的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過(guò)的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。