Phospho-p53 (Thr55) Rabbit Monoclonal Antibody
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價(jià)格
|
|
XY1696
|
Phospho-p53
(Thr55) Rabbit Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB
|
H
|
53.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:2,000
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this
Antibody
|
|
Name
|
Phospho-p53
(Thr55) Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
7157(Human)
|
|
SwissProt
|
P04637(Human)
|
|
Synonyms
|
BCC7; LFS1;
P53; TRP53
|
|
Category
|
Cell Cycle
|
|
Background
|
p53, a
DNA-binding, oligomerization domain- and transcription activation
domain-containing tumor suppressor, upregulates growth arrest and
apoptosis-related genes in response to stress signals, thereby influencing
programmed cell death, cell differentiation, and cell cycle control
mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the
cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase
that is upregulated in the presence of active p53, where it
poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between
latent and active DNA-binding conformations and is differentially activated
through posttranslational modifications, including phosphorylation and
acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids
110-286, can compromise energetically-favorable association with cis elements
and are implicated in several human cancers.
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
XY1696
|
Phospho-p53
(Thr55) Rabbit Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
Phospho-p53
(Thr55) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。