Ubiquitin Rabbit Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY1705
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Ubiquitin
Rabbit Monoclonal Antibody
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50μl
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618.00元
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來(lái)源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IF, IHC,
ICC, FC
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H, M, R
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55.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:50-1:200
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1:200-1:500
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1:50-1:200
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1:50-1:100
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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Ubiquitin
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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7314/7316/6233/7311(Human);
22187/22190/78294/22186(Mouse); 192255/100912032/64156(Rat)
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SwissProt
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P0CG47/P0CG48/P62979/P62987(Human);
P0CG49/P0CG50/P62983/P62984(Mouse); P0CG51/P62982/P62986(Rat)
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Synonyms
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FLJ25987;
MGC8385; Polyubiquitin B; RPS 27A; UBA 52; UBA 80; UBC; UBCEP 1; UBCEP 2
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Category
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Ubiquitin
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Background
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Ubiquitin is a
conserved polypeptide unit that plays an important role in the
ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to
another protein, or free (unanchored). When covalently bound, it is
conjugated to target proteins via an isopeptide bond either as a monomer
(monoubiquitin), a polymer linked via different Lys residues of the ubiquitin
(polyubiquitin chains) or a linear polymer linked via the initiator Met of
the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when
attached to a target protein, have different functions depending on the Lys
residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA
repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated
degradation) and in cell-cycle regulation; Lys-29-linked is involved in
lysosomal degradation; Lys-33-linked is involved in kinase modification;
Lys-48-linked is involved in protein degradation via the proteasome;
Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in
signaling processes leading to activation of the transcription factor
NF-kappa-B. This antibody reacts with ubiquitin, a polypeptide w/ Mr. of
approx. 8.5kD. It reacts with physiologically occurring conjugates of
ubiquitin and intracellular proteins. Specifically recognizes ubiquitinated
cytoplasmic inclusion bodies.
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY1705
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Ubiquitin
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說(shuō)明書(shū)
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1份
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保存條件:
Ubiquitin Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過(guò)的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過(guò)的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。